AB Vector - Active steroid/nuclear receptors
 
  Active steroid/nuclear receptors
   
 
In nature, all steroid receptors as well as some other nuclear receptors exist in complex with the Hsp90 molecular chaperone which is essential for native ligand-binding properties of the receptors (details). However, no such receptor/Hsp90 complexes were commercially available for drug screening in a purified form. AB Vector resolved this problem by co-expressing receptors with Hsp90 and other human molecular chaperones, i.e. Hsp40, Hsc70, Hop and p23, which are required to form and stabilize receptor/Hsp90 complexes (details), Fig. 1.
 
Fig. 1. A panel of purified complexes of GST-tagged ligand-binding domains of steroid/nuclear receptors with Hsp90.
Proteins were separated in 4-20% SDS-PAGE, stained with Coomassie blue and identified using mass-spectrometry.
Bands corresponding to the receptors are indicated by red arrows. PR-progesterone receptor, MR-mineralocorticoid receptor, AR-androgen receptor, GR-glucocorticoid receptor, PXR-pregnane X receptor, ERα-estrogen receptor α, AhR-arylhydrocarbon receptor, VDR-vitamin D receptor, PPARα-peroxisome proliferative activated receptor. Except for VDR and PPAR, receptors production in insect cells was supplemented with human molecular chaperones. Open the links above for the relevant products.


For details click on the Product name in the table below.

All products are readily available.
For large orders click here.
 
Purified receptor/Hsp90 complexes
Cat # Product name Specification Unit size Price*
SEa90 ERα-LBD Purified GST-tagged human Estrogen receptor alpha LBD in complex with Hsp90. Suitable for ligand-binding and coregulator displacement assays
50μg $980.00
SEb90 ERβ-LBD Purified GST-tagged human Estrogen receptor beta LBD in complex with Hsp90. Suitable for ligand-binding and coregulator displacement assays.
50μg $880.00
SG90 GR-LBD Purified GST-tagged human Glucocorticoid receptor LBD in complex with Hsp90. Suitable for ligand-binding and coregulator displacement assays.
50μg $1,100.00
S28 MR-LBD Purified GST-tagged human Mineralocorticoid receptor LBD in complex with Hsp90. Suitable for ligand-binding and coregulator displacement assays.
50μg $1,100.00
SP90 PR-LBD Purified GST-tagged human Progesterone receptor LBD in complex with Hsp90. Suitable for ligand-binding and coregulator displacement assays.
50μg $1,100.00
SPX90 PXR-LBD Purified GST-tagged human Pregnane X receptor in complex with Hsp90. Suitable for ligand-binding and coregulator displacement assays.
50μg $1,100.00
SC90 CAR Purified GST-tagged human Constitutive androstane receptor in complex with Hsp90. Suitable for ligand-binding and coregulator displacement assays.
50μg $1,100.00
SAh90 AhR-LBD Purified GST-tagged human Arylhydrocarbon receptor LBD in complex with Hsp90. Suitable for ligand-binding and coregulator displacement studies.
50μg $1,100.00
Purified nuclear receptors produced in insect cells
Cat # Product name Specification Unit size Price*
N1A2 THRB-LBD Purified GST-tagged human Thyroid hormone receptor beta ligand binding domain (NR1A2).
50μg $896.00
N1B1 RARA-LBD Purified GST-tagged human Retinoic acid receptor alpha ligand binding domain (NR1B1).
50μg $596.00
N1B2 RARB-LBD Purified GST-tagged human Retinoic acid receptor beta ligand binding domain (NR1B2).
50μg $495.00
N1B3 RARG-LBD Purified GST-tagged human Retinoic acid receptor gamma ligand binding domain (NR1B3).
50μg $596.00
N1C3 PPARG-LBD Purified GST-tagged human Peroxisome proliferator-activated receptor gamma ligand binding domain (NR1C3).
50μg $495.00
N1F1 RORA-LBD Purified GST-tagged human RAR-related orphan receptor alpha ligand binding domain (NR1F1).
50μg $896.00
N1H2 LXRB-LBD Purified GST-tagged human Liver X receptor beta ligand binding domain (NR1H2).               
50μg $596.00
N1H3 LXRA-LBD Purified GST-tagged human Liver X receptor alpha ligand binding domain (NR1H3).               
50μg $596.00
N1H4 FXR-LBD Purified GST-tagged human Farnesoid X receptor ligand binding domain (NR1H4).                             
50μg $395.00
N1I1 VDR-LBD Purified GST-tagged human Vitamin D receptor ligand binding domain (N1I1).                             
50μg $596.00
N2B1 RXRA-LBD Purified GST-tagged human Retinoid X receptor alpha ligand binding domain (NR2B1).               
50μg $495.00
N2B2 RXRB-LBD Purified GST-tagged human Retinoid X receptor beta ligand binding domain (NR2B2).               
50μg $495.00
N2B3 RXRG-LBD Purified GST-tagged human Retinoid X receptor gamma ligand binding domain (NR2B3).
50μg $596.00
N3A1 ERα-LBD Purified GST-tagged human Estrogen receptor alpha ligand binding domain (NR3A1).               
50μg $596.00
N3A2 ERβ-LBD Purified GST-tagged human Estrogen receptor beta ligand binding domain (NR3A2).               
50μg $495.00
N3B1 ESRRA-LBD Purified GST-tagged human Estrogen-related receptor alpha ligand binding domain (NR3B1).
50μg $980.00
N3B2 ESRRB-LBD Purified GST-tagged human Estrogen-related receptor beta ligand binding domain (NR3B2).
50μg $580.00
N3B3 ESRRG-LBD Purified GST-tagged human Estrogen-related receptor gamma ligand binding domain (NR3B3).
50μg $495.00
Recombinant baculoviruses for expression of steroid/nuclear receptors
Cat # Product name Specification Unit size Price*
S3 BV-ERα-LBD
Recombinant baculovirus stock
Expression of human GST-tagged human Estrogen receptor alpha LBD in insect cells.
1 ml, 108 p.f.u./ml $1,250.00
S15 BV-PR-LBD
Recombinant baculovirus stock
Expression of GST-tagged human Progersterone receptor LBD in insect cells.
1 ml, 108 p.f.u./ml $1,250.00
S21 BV-AR-LBD
Recombinant baculovirus stock
Expression of GST-tagged human Androgen receptor LBD in insect cells.
1 ml, 108 p.f.u./ml $1,250.00
S27 BV-MR-LBD
Recombinant baculovirus stock
Expression of GST-tagged human Mineralocorticoid receptor LBD in insect cells.
1 ml, 108 p.f.u./ml $1,250.00
S33 BV-GR-LBD
Recombinant baculovirus stock
Expression of GST-tagged human Glucocorticoid receptor LBD in insect cells.
1 ml, 108 p.f.u./ml $1,250.00
S35 BV-GR
Recombinant baculovirus stock
Expression of human Glucocorticoid receptor full length in insect cells.
1 ml, 108 p.f.u./ml $1,250.00
S36 BV-PXR-LBD
Recombinant baculovirus stock
Expression of GST-tagged human Pregnane X receptor LBD in insect cells.
1 ml, 108 p.f.u./ml $1,250.00
S41 BV-AhR-LBD
Recombinant baculovirus stock
Expression of GST-tagged human Aryl hydrocarbcarbon receptor LBD in insect cells.
1 ml, 108 p.f.u./ml $1,250.00
Transcription factors
Cat # Product name Specification Unit size Price*
BV53 BV-p53
Recombinant baculovirus stock
Expression of GST-tagged human p53 (wild type) in insect cells.
1 ml, 108 p.f.u./ml $980.00
53S P53
Purified protein
Purified GST-tagged human p53 (wild type). Suitable for protein-protein interaction studies, WB and ELISA.
10μg $220.00
53L P53
Purified protein
Purified GST-tagged human p53 (wild type). Suitable for protein-protein interaction studies, WB and ELISA.
50μg $780.00
  *Go to PRODUCTS to order online. Alternatively, email an order as pdf attachment to info@abvector.com, or send it by Fax to 1-760-454-2465 or call 1-866-683-2867 to order by phone.


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