AB Vector - RARB-LBD™
 
    RARB-LBD™ Cat. #N1B2
  Purified GST-tagged human Retinoic acid receptor beta LBD
 
  Retinoic acid receptor beta (RAR-beta or RARB) is also known as NR1B2 (nuclear receptor subfamily 1, group B, member 2). GST-tagged Retinoic acid receptor beta ligand-binding domain (RARB-LBD) consists of a 218 a.a. GST tag (Schistosoma japonicum Glutathione S-Transferase, Acc# 1M99_A), a 14 a.a. flexible linker and a 281 a.a. RARB-LBD, Acc# AAH60794.1, amino acids 168-448 (details).

GST tagged RARB-LBD was expressed in baculovirus-infected insect cells and purified using affinity chromatography (Fig. 1). RARB-LBD was produced in the absence of detergents and exogenous ligands to ensure advanced performance in ligand-binding assays and coregulator displacement studies.

  Storage buffer
20 mM Na-P pH 8.0, 40 mM KCl,
20 mM Na2MoO4, 7 mM reduced
glutathione, 15% glycerol.

Purity >90%

Applications
Ligand binding and coactivator
peptide recruitment assays.

Handling upon arrival
Immediately store at -80C.
Avoid freeze-thaw cycles.
While working, please keep
sample on ice.






  Fig. 1. SDS-PAGE analysis of purified human Retinoic
  acid receptor beta.
  Proteins were separated in 4-20% SDS-PAGE and stained
  with Coomassie blue.
          1.        RARB-LBD.
          2.        Low range molecular weight marker (Bio-Rad).


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