| Purified GST-tagged human Retinoic acid receptor beta LBD |
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Retinoic acid receptor beta (RAR-beta or RARB) is also known as NR1B2 (nuclear receptor subfamily 1, group B, member 2). GST-tagged Retinoic acid receptor beta ligand-binding domain (RARB-LBD) consists of a 218 a.a. GST tag (Schistosoma japonicum Glutathione S-Transferase, Acc# 1M99_A), a 14 a.a. flexible linker and a 281 a.a. RARB-LBD, Acc# AAH60794.1, amino acids 168-448 (details). GST tagged RARB-LBD was expressed in baculovirus-infected insect cells and purified using affinity chromatography (Fig. 1). RARB-LBD was produced in the absence of detergents and exogenous ligands to ensure advanced performance in ligand-binding assays and coregulator displacement studies. |
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Storage buffer 20 mM Na-P pH 8.0, 40 mM KCl, 20 mM Na2MoO4, 7 mM reduced glutathione, 15% glycerol. Purity >90% Applications Ligand binding and coactivator peptide recruitment assays. Handling upon arrival Immediately store at -80°C. Avoid freeze-thaw cycles. While working, please keep sample on ice. |
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Fig. 1. SDS-PAGE analysis of purified human Retinoic acid receptor beta. Proteins were separated in 4-20% SDS-PAGE and stained with Coomassie blue. 1. RARB-LBD. 2. Low range molecular weight marker (Bio-Rad). |