|Purified GST-tagged human Retinoic acid receptor beta LBD
Retinoic acid receptor beta (RAR-beta or RARB) is also known as NR1B2 (nuclear receptor subfamily 1, group B, member 2). GST-tagged Retinoic acid receptor beta ligand-binding domain (RARB-LBD) consists of a 218 a.a. GST tag (Schistosoma japonicum Glutathione S-Transferase, Acc# 1M99_A), a 14 a.a. flexible linker and a 281 a.a. RARB-LBD, Acc# AAH60794.1, amino acids 168-448 (details).
GST tagged RARB-LBD was expressed in baculovirus-infected insect cells and purified using affinity chromatography (Fig. 1). RARB-LBD was produced in the absence of detergents and exogenous ligands to ensure advanced performance in ligand-binding assays and coregulator displacement studies.
20 mM Na-P pH 8.0, 40 mM KCl,
20 mM Na2MoO4, 7 mM reduced
glutathione, 15% glycerol.
Ligand binding and coactivator
peptide recruitment assays.
Handling upon arrival
Immediately store at -80°C.
Avoid freeze-thaw cycles.
While working, please keep
sample on ice.
Fig. 1. SDS-PAGE analysis of purified human Retinoic
acid receptor beta.
Proteins were separated in 4-20% SDS-PAGE and stained
with Coomassie blue.
2. Low range molecular weight marker (Bio-Rad).