AB Vector - ESRRB-LBD™
    ESRRB-LBD™ Cat. #N3B2
  Purified GST-tagged human Estrogen-related receptor beta LBD
  Estrogen-related receptor beta (ESRRB, ERRβ, ERR2) is also known as nuclear receptor subfamily 3, group B, member 2 (NR3B2). GST-tagged Estrogen-related receptor beta ligand-binding domain (ESRRB-LBD) consists of a 218 a.a. GST tag (Schistosoma japonicum Glutathione S-Transferase, Acc# 1M99_A), a 16 a.a. flexible linker and a 304 a.a. ESRRB-LBD, Acc# AAI31518.1 (details).

GST tagged ESRRB-LBD was expressed in baculovirus-infected insect cells and purified using affinity chromatography (Fig. 1). ESRRB-LBD was produced in the absence of detergents and exogenous ligands to ensure advanced performance in ligand-binding assays and coregulator displacement studies.

  Storage buffer
50 mM Tris-HCl pH 8.0, 150 mM NaCl,
0.5 mM EDTA, 3 mM Dithiothreitol,
7 mM reduced glutathione, 20% glycerol.

Purity >80%

Ligand binding and coactivator
peptide recruitment assays.

Handling upon arrival
Immediately store at -80C.
Avoid freeze-thaw cycles.
While working, please keep
sample on ice.

  Fig. 1. SDS-PAGE analysis of purified Estrogen related
  receptor beta LBD.
  Proteins were separated in 4-20% SDS-PAGE and
  stained with Coomassie blue.
          1.        ESRRB-LBD.
          2.        Low range molecular weight marker (Bio-Rad).