AB Vector - ERβ-LBD™
 
    ERβ-LBD™ Cat. #N3A2
  Purified GST-tagged human Estrogen receptor beta LBD
 
  Estrogen receptor beta (ERβ) is also known as NR3A2 (nuclear receptor subfamily 3, group A, member 2). GST-tagged Estrogen receptor beta ligand-binding domain (ERβ-LBD) consists of a 218 a.a. GST tag (Schistosoma japonicum Glutathione S-Transferase, Acc# 1M99_A), a 30 a.a. flexible linker and a 346 a.a. ERβ-LBD, Acc# NP_001428, amino acids 243-530 (details).

GST tagged ERβ-LBD was expressed in baculovirus-infected insect cells and purified using affinity chromatography (Fig. 1). ERβ-LBD was produced in the absence of detergents and exogenous ligands to ensure advanced performance in ligand-binding assays and coregulator displacement studies.

  Storage buffer
50 mM Tris-HCl pH 8.0, 150 mM NaCl,
0.5 mM EDTA, 20 mM Na2MoO4,
7 mM reduced glutathione, 20% glycerol.

Purity >90%

Applications
Ligand binding and coactivator
peptide recruitment assays.

Handling upon arrival
Immediately store at -80C.
Avoid freeze-thaw cycles.
While working, please keep
sample on ice.






  Fig. 1. SDS-PAGE analysis of purified Estrogen receptor
  beta LBD.
  Proteins were separated in 4-20% SDS-PAGE and stained
  with Coomassie blue.
          1.        ERβ-LBD.
          2.        Low range molecular weight marker (Bio-Rad).


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