|Purified GST-tagged human Liver X receptor beta LBD
Liver X receptor beta (LXR-β or LXRB) is encoded by the NR1H2 gene (nuclear receptor subfamily 1, group H, member 2). GST-tagged Liver X receptor beta ligand-binding domain (LXRB-LBD) consists of a 218 a.a. GST tag (Schistosoma japonicum Glutathione S-Transferase, Acc# 1M99_A), a 13 a.a. flexible linker and a 266 a.a. LXRB-LBD, Acc# AAH74500.1, amino acids 196-461 (details).
GST tagged LXRB-LBD was expressed in baculovirus-infected insect cells and purified using affinity chromatography (Fig. 1). LXRB-LBD was produced in the absence of detergents and exogenous ligands to ensure advanced performance in ligand-binding assays and coregulator displacement studies.
20 mM Na-P pH 8.0, 40 mM KCl,
7 mM reduced glutathione,
Ligand binding and coactivator
peptide recruitment assays.
Handling upon arrival
Immediately store at -80°C.
Avoid freeze-thaw cycles.
While working, please keep
sample on ice.
Fig. 1. SDS-PAGE analysis of purified human human Liver X
receptor beta LBD.
Proteins were separated in 4-20% SDS-PAGE and stained
with Coomassie blue.
2. Low range molecular weight marker (Bio-Rad).