|Purified GST-tagged human Peroxisome proliferator-activated receptor gamma LBD
Peroxisome proliferator-activated receptor gamma (PPAR-γ or PPARG) is also known as the glitazone receptor, or NR1C3 (nuclear receptor subfamily 1, group C, member 3). GST-tagged Peroxisome proliferator-activated receptor gamma ligand-binding domain (PPARG-LBD) consists of a 218 a.a. GST tag (Schistosoma japonicum Glutathione S-Transferase, Acc# 1M99_A), a 13 a.a. flexible linker and a 277 a.a. PPARG-LBD, Acc# AAH06811.1, amino acids 201-477 (details).
GST tagged PPARG-LBD was expressed in baculovirus-infected insect cells and purified using affinity chromatography (Fig. 1). PPARG-LBD was produced in the absence of detergents and exogenous ligands to ensure advanced performance in ligand-binding assays and coregulator displacement studies.
50 mM Tris-HCl pH 8.0, 150 mM NaCl,
0.5 mM EDTA, 3 mM Dithiothreitol,
7 mM reduced glutathione, 20% glycerol.
Ligand binding and coactivator
peptide recruitment assays.
Handling upon arrival
Immediately store at -80°C.
Avoid freeze-thaw cycles.
While working, please keep
sample on ice.
Fig. 1. SDS-PAGE analysis of purified Human Peroxisome
proliferator-activated receptor gamma ligand-binding
Proteins were separated in 4-20% SDS-PAGE and stained
with Coomassie blue.
2. Low range molecular weight marker (Bio-Rad).
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