|Purified GST-tagged human Vitamin D receptor LBD
GST-tagged Vitamin D receptor ligand-binding domain (VDR-LBD) consists of a 218 a.a. GST tag (Schistosoma japonicum Glutathione S-Transferase, Acc# 1M99_A), a 13 a.a. flexible linker and a 322 a.a. VDR-LBD, Acc# NP_000367.1, amino acids 105-427 (details).
Human Vitamin D receptor ligand-binding domain (VDR-LBD) was produced in insect cells infected with a recombinant baculovirus which encodes GST-tagged human Vitamin D receptor.
GST tagged VDR-LBD was purified using affinity chromatography (Fig. 1). VDR-LBD was produced in the absence of detergents and exogenous ligands to ensure advanced performance in ligand-binding assays and coregulator displacement studies.
20 mM Na-P pH 8.0, 40 mM KCl,
20 mM Na2MoO4, 7 mM reduced
glutathione, 15% glycerol.
Ligand binding and coactivator
peptide recruitment assays.
Handling upon arrival
Immediately store at -80°C.
Avoid freeze-thaw cycles.
While working, please keep
sample on ice.
Fig. 1. SDS-PAGE analysis of purified human Vitamin D
Proteins were separated in 4-20% SDS-PAGE and stained with
Coomassie blue. Identity of VDR-LBD was confirmed using
mass-spectrorometry. Open the link above for amino acid
1. Vitamin D receptor LBD.
2. Low range molecular weight marker (Bio-Rad).