AB Vector - VDR-LBD™
 
    VDR-LBD™ Cat. #N1I1
  Purified GST-tagged human Vitamin D receptor LBD
 
  GST-tagged Vitamin D receptor ligand-binding domain (VDR-LBD) consists of a 218 a.a. GST tag (Schistosoma japonicum Glutathione S-Transferase, Acc# 1M99_A), a 13 a.a. flexible linker and a 322 a.a. VDR-LBD, Acc# NP_000367.1, amino acids 105-427 (details).

Human Vitamin D receptor ligand-binding domain (VDR-LBD) was produced in insect cells infected with a recombinant baculovirus which encodes GST-tagged human Vitamin D receptor.

GST tagged VDR-LBD was purified using affinity chromatography (Fig. 1). VDR-LBD was produced in the absence of detergents and exogenous ligands to ensure advanced performance in ligand-binding assays and coregulator displacement studies.

  Storage buffer
20 mM Na-P pH 8.0, 40 mM KCl,
20 mM Na2MoO4, 7 mM reduced
glutathione, 15% glycerol.

Purity >90%

Applications
Ligand binding and coactivator
peptide recruitment assays.

Handling upon arrival
Immediately store at -80C.
Avoid freeze-thaw cycles.
While working, please keep
sample on ice.






  Fig. 1. SDS-PAGE analysis of purified human Vitamin D
  receptor LBD.
  Proteins were separated in 4-20% SDS-PAGE and stained with
  Coomassie blue. Identity of VDR-LBD was confirmed using
  mass-spectrorometry. Open the link above for amino acid
  sequence coverage.
          1.        Vitamin D receptor LBD.
          2.        Low range molecular weight marker (Bio-Rad).


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