VDR-LBD™	Cat. #N1I1

Purified GST-tagged human Vitamin D receptor LBD

GST-tagged Vitamin D receptor ligand-binding domain (VDR-LBD) consists of a 218 a.a. GST tag (Schistosoma japonicum Glutathione S-Transferase, Acc# 1M99_A), a 13 a.a. flexible linker and a 322 a.a. VDR-LBD, Acc# NP_000367.1, amino acids 105-427 (details). Human Vitamin D receptor ligand-binding domain (VDR-LBD) was produced in insect cells infected with a recombinant baculovirus which encodes GST-tagged human Vitamin D receptor. GST tagged VDR-LBD was purified using affinity chromatography (Fig. 1). VDR-LBD was produced in the absence of detergents and exogenous ligands to ensure advanced performance in ligand-binding assays and coregulator displacement studies.

Storage buffer 20 mM Na-P pH 8.0, 40 mM KCl, 20 mM Na2MoO4, 7 mM reduced glutathione, 15% glycerol. Purity >90% Applications Ligand binding and coactivator peptide recruitment assays. Handling upon arrival Immediately store at -80°C. Avoid freeze-thaw cycles. While working, please keep sample on ice.

Fig. 1. SDS-PAGE analysis of purified human Vitamin D   receptor LBD.   Proteins were separated in 4-20% SDS-PAGE and stained with   Coomassie blue. Identity of VDR-LBD was confirmed using   mass-spectrorometry. Open the link above for amino acid   sequence coverage.           1.        Vitamin D receptor LBD.           2.        Low range molecular weight marker (Bio-Rad).