BV-PXR-LBD™ is a recombinant baculovirus which provides for high level expression of GST-tagged human pregnane X receptor, PXR, NR1I2 (Acc# AAH17304.2) ligand binding domain (amino acids 201-433). A GST tag is fused to the N-terminus of the ligand binding domain of the receptor. The fusion protein is expressed under control of a very strong polyhedrin promoter using pAB-GST™ vector.
If you have not worked with baculovirus expression system, also referred to as baculovirus expression vector system or BEVS, you can get a quick update at TECHNOLOGY. Go to BACULOVIRUS TUTORIAL for simple on-line instructions on baculovirus transfection, propagation of recombinant baculoviruses and recombinant baculovirus protein expression studies.
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