|pAB-GST™ plasmid transfer vector is designed for expression of proteins with N-terminal glutathione S-transferase (GST) tag (Smith D.B. & Johnson K.S., Gene, 15:31-40, 1988). A PCR fragment encoding a protein of interest can be cloned in lieu of the polyhedrin promoter and GST tag using any of the unique restriction endonuclease sites (BamHI, EcoRI, SmaI, NotI, PstI, BglII) located in the MCS. Proteins are expressed under control of strong very late polyhedrin promoter in the polyhedrin site of baculovirus DNA. Affinity purification of the expressed proteins can be achieved using Glutathione Sepharose™ 4B resin (Amersham Biosciences). If desired, GST tag can be removed with PreScission™ protease which cleaves at specific site (LEVLFQGPL) encoded at nucleotides 4798-4824 of the vector.|
|Sepharose and PreScission are trademarks of Amersham Biosciences.
The above plasmid transfer vector is compatible with linearized baculovirus DNA from several other suppliers, e.g. BacMagic™, BacMagic™-2, BacMagic™-3, BacVector™-1000, BacVector™-2000, BacVector™-3000 (Novagen); BaculoGold™, BaculoGold™ bright (BD Pharmingen); flashBACGOLD™, flashBACULTRA™, flashBAC™ (OET); BacPAK6™ (Clontech); Sapphire™ (Orbigen). They are not compatible with Bac-to-Bac® and BaculoDirect™ DNA from Invitrogen. BacMagic™, BacMagic™-2, BacMagic™-3, BacVector™-1000, BacVector™-2000, BacVector™-3000 are trademarks of Novagen; BaculoGold™, BaculoGold™ bright are trademarks of BD Pharmingen; flashBACGOLD™, flashBACULTRA™, flashBAC™ are trademarks of OET; BacPAK6™ is a trademark of Clontech; Sapphire™ is a trademark of Orbigen, Bac-to-Bac® and BaculoDirect™ are trademarks of Invitrogen.
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