AB Vector - p53

    p53 Cat. #53S,#53L
 
  Purified GST-tagged human p53

  GST-tagged human P53 consists of a 218 a.a. GST tag (Schistosoma japonicum Glutathione S-Transferase, Acc# 1M99_A), a 20 a.a. flexible linker and an abundant 393 a.a. wild-type p53 variant, Acc# NP_000537.3 (details).

Human p53 was produced in insect cells infected with a BV-p53 recombinant baculovirus which encodes GST-tagged human p53 and was purified to near homogeneity using affinity chromatography.

  Storage buffer
50 mM Tris-HCl pH 8.0, 150 mM
NaCl, 2 mM DTT, 15% glycerol.

Purity >90%

Applications
Protein-protein interaction studies,
WB and ELISA.

Handling upon arrival
Immediately store at -80C.
Avoid freeze-thaw cycles.






  Fig. 1. SDS-PAGE analysis of purified human p53 in complex
  with Hsp90.
  Proteins were separated in 4-20% SDS-PAGE and stained with
  Coomassie blue.
          1.        GST-tagged p53.
          2.        Low range molecular weight marker (Bio-Rad).


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