|Purified GST-tagged human p53
GST-tagged human P53 consists of a 218 a.a. GST tag (Schistosoma japonicum Glutathione S-Transferase, Acc# 1M99_A), a 20 a.a. flexible linker and an abundant 393 a.a. wild-type p53 variant, Acc# NP_000537.3 (details).
Human p53 was produced in insect cells infected with a BV-p53™ recombinant baculovirus which encodes GST-tagged human p53 and was purified to near homogeneity using affinity chromatography.
50 mM Tris-HCl pH 8.0, 150 mM
NaCl, 2 mM DTT, 15% glycerol.
Protein-protein interaction studies,
WB and ELISA.
Handling upon arrival
Immediately store at -80°C.
Avoid freeze-thaw cycles.
Fig. 1. SDS-PAGE analysis of purified human p53 in complex
Proteins were separated in 4-20% SDS-PAGE and stained with
1. GST-tagged p53.
2. Low range molecular weight marker (Bio-Rad).