| Purified GST-tagged human Retinoid X receptor alpha LBD |
||
|
Retinoid X receptor alpha (RXR-alpha) is also known as NR2B1 (nuclear receptor subfamily 2, group B, member 1). GST-tagged Retinoid X receptor alpha ligand-binding domain (RXRA-LBD) consists of a 218 a.a. GST tag (Schistosoma japonicum Glutathione S-Transferase, Acc# 1M99_A), a 13 a.a. flexible linker and a 260 a.a. RXRA-LBD, Acc# AAI10999.1, amino acids 203-462 aa (details). GST tagged RXRA-LBD was expressed in baculovirus-infected insect cells and purified using affinity chromatography (Fig. 1). RXRA-LBD was produced in the absence of detergents and exogenous ligands to ensure advanced performance in ligand-binding assays and coregulator displacement studies. |
||
|
Storage buffer 50 mM Tris-HCl pH 8.0, 150 mM NaCl, 3 mM DTT, 7mM reduced glutathione, 0.5 mM EDTA, 20% glycerol. Purity >90% Applications Ligand binding and coactivator peptide recruitment assays. Handling upon arrival Immediately store at -80°C. Avoid freeze-thaw cycles. While working, please keep sample on ice. |
|
Fig. 1. SDS-PAGE analysis of purified Retinoid X receptor alpha LBD. Proteins were separated in 4-20% SDS-PAGE and stained with Coomassie blue. 1. RXRA-LBD. 2. Low range molecular weight marker (Bio-Rad). |