|Purified GST-tagged human Retinoid X receptor alpha LBD
Retinoid X receptor alpha (RXR-alpha) is also known as NR2B1 (nuclear receptor subfamily 2, group B, member 1). GST-tagged Retinoid X receptor alpha ligand-binding domain (RXRA-LBD) consists of a 218 a.a. GST tag (Schistosoma japonicum Glutathione S-Transferase, Acc# 1M99_A), a 13 a.a. flexible linker and a 260 a.a. RXRA-LBD, Acc# AAI10999.1, amino acids 203-462 aa (details).
GST tagged RXRA-LBD was expressed in baculovirus-infected insect cells and purified using affinity chromatography (Fig. 1). RXRA-LBD was produced in the absence of detergents and exogenous ligands to ensure advanced performance in ligand-binding assays and coregulator displacement studies.
50 mM Tris-HCl pH 8.0, 150 mM NaCl,
3 mM DTT, 7mM reduced glutathione,
0.5 mM EDTA, 20% glycerol.
Ligand binding and coactivator
peptide recruitment assays.
Handling upon arrival
Immediately store at -80°C.
Avoid freeze-thaw cycles.
While working, please keep
sample on ice.
Fig. 1. SDS-PAGE analysis of purified Retinoid X
receptor alpha LBD.
Proteins were separated in 4-20% SDS-PAGE and stained
with Coomassie blue.
2. Low range molecular weight marker (Bio-Rad).