AB Vector - ERα-LBD™
 
    ERα-LBD™ Cat. #N3A1
  Purified GST-tagged human Estrogen receptor alpha LBD
 
  Estrogen receptor alpha (ERα) is also known as NR3A1 (nuclear receptor subfamily 3, group A, member 1). GST-tagged Estrogen receptor alpha ligand-binding domain (ERα-LBD) consists of a 218 a.a. GST tag (Schistosoma japonicum Glutathione S-Transferase, Acc# 1M99_A), a 30 a.a. flexible linker and a 346 a.a. ERα-LBD, Acc# NP_000116 amino acids 250-595 (details).

GST tagged ERα-LBD was expressed in baculovirus-infected insect cells and purified using affinity chromatography (Fig. 1). ERα-LBD was produced in the absence of detergents and exogenous ligands to ensure advanced performance in ligand-binding assays and coregulator displacement studies.

  Storage buffer
50 mM Tris-HCl pH 8.0, 150 mM NaCl,
0.5 mM EDTA, 20 mM Na2MoO4,
7 mM reduced glutathione, 20% glycerol.

Purity >90%

Applications
Ligand binding and coactivator
peptide recruitment assays.

Handling upon arrival
Immediately store at -80C.
Avoid freeze-thaw cycles.
While working, please keep
sample on ice.






  Fig. 1. SDS-PAGE analysis of purified Estrogen receptor
  alpha LBD.
  Proteins were separated in 4-20% SDS-PAGE and stained
  with Coomassie blue.
          1.        ERα-LBD.
          2.        Low range molecular weight marker (Bio-Rad).


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