|GFP tag vector|
Our pVL-GFP vector allows expression of target proteins in-fusion with the C-terminal GFP tag. The GFP tag is widely used for visualization and isolation of protein complexes, studies of protein-protein interactions, protein trafficking within the cells, and mechanisms of protein degradation (Cristea et al., Mol Cell Proteomics, 4:1933-41, 2005; Michaelson and Philips, Methods Enzymol, 406:296-315, 2006; Hayes et al., Cancer Lett., 206:129-35, 2004 ; Takeuchi et al., EMBO J., 26:123-31, 2007).
Especially useful is the ability of the GFP tag to misfold and loose its fluorescence if its fusion partner is misfolded. This property was used for selection of target protein mutants with improved solubility (Van den Berg et at, J Biotechnol, 121:291-8, 2006), for finding soluble proteins derived from randomly fragmented cDNAs (Nakayama and Ohara, Biochem Biophys Res Commun, 312:825-30, 2003), and in studies of protein aggregation (Schrodel and de Marco, BMC Biochem, 6:10, 2005).
Of particular interest are studies of GFP fusion aggregates with proteins related to protein folding diseases (Krobitsch and Lindquist, Proc Natl Acad Sci U S A, 97:1589-94, 2000; Wang et al., Hum Mol Genet., 14:3673-84, 2005; Pandey et al., Exp Neurol., 197:515-20, 2006; Jiang et al., J Biol Chem., 282:7912-20, 2007). Such fusions could be used for high throughput screening of compounds that inhibit aggregation of these important drug targets (Gazit, ASC Chemical Biology, 1:417-19, 2006). AB Vector offers some protein folding diseases targets in-fusion with GFP.
The pVL-GFP plasmid transfer vector can be combined with any of the listed below linearized baculovirus DNA vectors that do not encode GFP, i.e. ProEasy™, ProFold™-PDI, and ProFold™-0. Click on TECHNOLOGY for more information on baculovirus expression system. Go to BACULOVIRUS TUTORIAL for simple on-line instructions on baculovirus transfection, propagation of recombinant baculoviruses and recombinant baculovirus protein expression studies.
*pVL-GFP plasmid transfer vector is compatible with linearized baculovirus DNA from several other suppliers, e.g. BacMagic™, BacMagic™-2, BacMagic™-3, BacVector™-1000, BacVector™-2000, BacVector™-3000 (Novagen); BaculoGold™, BaculoGold™ bright (BD Pharmingen); flashBACGOLD™, flashBACULTRA™, flashBAC™ (OET); BacPAK6™ (Clontech); Sapphire™ (Orbigen). They are not compatible with Bac-to-Bac® and BaculoDirect™ DNA from Invitrogen. BacMagic™, BacMagic™-2, BacMagic™-3, BacVector™-1000, BacVector™-2000, BacVector™-3000 are trademarks of Novagen; BaculoGold™, BaculoGold™ bright are trademarks of BD Pharmingen; flashBACGOLD™, flashBACULTRA™, flashBAC™ are trademarks of OET; BacPAK6™ is a trademark of Clontech; Sapphire™ is a trademark of Orbigen, Bac-to-Bac® and BaculoDirect™ are trademarks of Invitrogen.
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|If you have not worked with recombinant baculoviruses, you can get a quick update at TECHNOLOGY. Go to BACULOVIRUS TUTORIAL for simple on-line instructions on baculovirus transfection, propagation of recombinant baculoviruses and recombinant baculovirus protein expression studies.|
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|Commercial use of GFP reporter requires licensing.|