BV-SOD1-GFP™ is a recombinant baculovirus which provides for high level expression of GFP-tagged human SOD1 (Acc# CAG46542). A GFP tag (Aequorea victoria green fluorescent protein) is fused to the C-terminus of full size SOD1 via flexible Ile-Ser-Gly-Gly-Gly-Ser-Gln-Lys spacer. The fusion protein is expressed under control of a very strong p10 promoter and exhibits strong green fluorescence (GFP reporter).
If you have not worked with baculovirus expression system, also referred to as baculovirus expression vector system or BEVS, you can get a quick update at TECHNOLOGY. Go to BACULOVIRUS TUTORIAL for simple on-line instructions on baculovirus transfection, propagation of recombinant baculoviruses and recombinant baculovirus protein expression studies.
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