G protein-coupled receptors (GPCRs)
	G protein-coupled receptors (GPCRs) are among the most important drug targets, and sales from drugs targeting GPCRs exceed sales from drugs targeting other protein classes (details). Most of the GPCRs used for drug screening and structural studies are produced using either mammalian or baculovirus protein expression systems. GlaxoSmithKline scientists expressed 16 GPCRs using a baculovirus system and concluded that overall performance was superior to other systems (Akermoun M. et al., Protein Expr. Purif., 44: 65-74, 2005). AB Vector offers the largest selection of recombinant baculoviruses for producing the G protein-coupled receptors of top priority. We routinely provide 1ml stocks, but larger amounts could be made available contingent on the demand.
	Medicines targeting Histamine receptors are used for treating allergies-Claritin (Schering Plough), Allegra (Aventis), and for treating ulcers-Zantac (GlaxoSmithKline), Pepcid (Merck). AB Vector provides recombinant baculovirus stocks for expressing all Histamine receptors.
	AB Vector also provides recombinant baculovirus stocks for expressing most of the Serotonin and Adrenergic receptors. Medicines targeting Serotonin receptors are used for treating migraines-Imitrex (GlaxoSmithKline), anxiety-BuSpar (Bristol-Myers Squibb), psychosis- Risperdal (Johnson & Johnson), schizophrenia-Zyprexa (Eli Lilly). Medicines targeting Adrenergic receptors are used for treating congestive heart failure-Coreg (GlaxoSmithKline) and asthma-Severent (GlaxoSmithKline).
	AB vector also provides Dopamine, Muscarinic and Angiotensin receptors of prime importance. Requip (GlaxoSmithKline) which is used for treating Parkinson's disease targets the Dopamine D2 receptor. Atrovent (Boehringer Ingelheim) which is used for treating COPD targets the Muscarinic M1 and M2 receptors. Cozaar (Merck) which is used for treating hypertension targets the angiotensin II receptor.
	GPCRs have proven to be eminently suitable for therapeutic intervention, in part due to the fact that they are easily accessible to the drugs on the cell surface. Among approximately 500 drugs on the market about 50% target GPCRs. Unfortunately, GPCRs are among the most difficult proteins to produce. Due to difficulties in producing native GPCRs in sufficient quantities, not a single structure of a therapeutically relevant GPCR was solved, thus impeding protein-structure based drug design for these targets.
	AB vector believes that GPCR expression levels are low due to insufficient quantities of protein folding factors specific to GPCRs. For example, a mammalian or baculovirus system is universally expected to produce any human protein. However, many proteins misfold and are either degraded or produced in the form of biologically inactive aggregates. The root of the problem is that expression systems are geared for producing target proteins in much higher than physiological amounts without providing sufficient quantities of corresponding protein folding partners.
	AB Vector succeeded in a many-fold improvement in the yield of steroid receptors by providing an ensemble of protein folding factors specific to these receptors (Multi-chaperone systems). We hope to achieve similar improvement in the production of GPCRs by providing ensembles of protein folding factors specific to GPCRs. Please inquire at info@abvector.com if you are an expert in GPCR- ligand-binding studies or GPCR structure and would like to collaborate.
	Relevant products
	Histamine receptors Cat # Product Specification Unit size Price* GH41 BV-HRH1 Recombinant baculovirus stock Expression of Histamine receptor (HRH1) 1 ml, 108 p.f.u./ml $499.00 GH45 BV-HRH2 Recombinant baculovirus stock Expression of Histamine receptor (HRH2) 1 ml, 108 p.f.u./ml $499.00 GH49 BV-HRH3 Recombinant baculovirus stock Expression of Histamine receptor (HRH3) 1 ml, 108 p.f.u./ml $499.00 GH53 BV-HRH4 Recombinant baculovirus stock Expression of Histamine receptor (HRH4) 1 ml, 108 p.f.u./ml $499.00 Serotonin receptors Cat # Product Specification Unit size Price* GS35 BV-HTR1D Recombinant baculovirus stock Expression of 5-hydroxytryptamine (serotonin) receptor 1D (HTR1D) 1 ml, 108 p.f.u./ml $499.00 GS37 BV-HTR1F™ Recombinant baculovirus stock Expression of 5-hydroxytryptamine (serotonin) receptor 1F (HTR1F) 1 ml, 108 p.f.u./ml $499.00 GS39 BV-HTR2A Recombinant baculovirus stock Expression of 5-hydroxytryptamine (serotonin) receptor 2A (HTR2A) 1 ml, 108 p.f.u./ml $499.00 GS43 BV-HTR2B Recombinant baculovirus stock Expression of 5-hydroxytryptamine (serotonin) receptor 2B (HTR2B) 1 ml, 108 p.f.u./ml $499.00 GS47 BV-HTR2B Recombinant baculovirus stock Expression of 5-hydroxytryptamine (serotonin) receptor 2C (HTR2C 1 ml, 108 p.f.u./ml $499.00 GS51 BV-HTR5A Recombinant baculovirus stock Expression of 5-hydroxytryptamine (serotonin) receptor 5A (HTR5A) 1 ml, 108 p.f.u./ml $499.00 GS55 BV-HTR7B Recombinant baculovirus stock Expression of 5-hydroxytryptamine (serotonin) receptor 7B (HTR7B) 1 ml, 108 p.f.u./ml $499.00 Adrenergic receptors Cat # Product Specification Unit size Price* GA3 BV-ADRA1A™ Recombinant baculovirus stock Expression of Adrenergic Receptor alpha 1A (ADRA1A) 1 ml, 108 p.f.u./ml $499.00 GA7 BV-ADRA1B™ Recombinant baculovirus stock Expression of Adrenergic Receptor alpha 1B (ADRA1B) 1 ml, 108 p.f.u./ml $499.00 GA11 BV-ADRA1D™ Recombinant baculovirus stock Expression of Adrenergic Receptor alpha 1D (ADRA1D) 1 ml, 108 p.f.u./ml $499.00 GA15 BV-ADRA2A™ Recombinant baculovirus stock Expression of Adrenergic Receptor alpha 2A (ADRA2A 1 ml, 108 p.f.u./ml $499.00 GA19 BV-ADRA2C™ Recombinant baculovirus stock Expression of Adrenergic Receptor alpha 2C (ADRA2C) 1 ml, 108 p.f.u./ml $499.00 GA23 BV-ADRB2™ Recombinant baculovirus stock Expression of Adrenergic Receptor beta 2 (ADRB2) 1 ml, 108 p.f.u./ml $499.00 Dopamine receptors Cat # Product Specification Unit size Price* GD3 BV-DRD1 Recombinant baculovirus stock Expression of Dopamine receptor 1 (DRD1) 1 ml, 108 p.f.u./ml $499.00 GD5 BV-DRD1 Recombinant baculovirus stock Expression of Dopamine receptor 2, transcript variant 1 (DRD2) 1 ml, 108 p.f.u./ml $499.00 Muscarinic receptors Cat # Product Specification Unit size Price* GM1 BV-CHRM1 Recombinant baculovirus stock Expression of Muscarinic acetylcholine receptor 1 (CHRM1) 1 ml, 108 p.f.u./ml $499.00 Angiotensin receptors Cat # Product Specification Unit size Price* GAT1 BV-AGTR1 Recombinant baculovirus stock Expression of angiotensin II receptor (AGTR1) 1 ml, 108 p.f.u./ml $499.00
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