|Purified GST-tagged human RAR-related orphan receptor alpha LBD
RAR-related orphan receptor alpha (ROR-alpha or RORA), also known as NR1F1 (nuclear receptor subfamily 1, group F, member 1). GST-tagged RAR-related orphan receptor alpha ligand-binding domain (RORA-LBD) consists of a 218 a.a. GST tag (Schistosoma japonicum Glutathione S-Transferase, Acc# 1M99_A), a 13 a.a. flexible linker and a 262 a.a. RORA-LBD, Acc# AAH08831.1, amino acids 262-523 (details).
GST tagged RORA-LBD was expressed in baculovirus-infected insect cells and purified using affinity chromatography (Fig. 1). RORA-LBD was produced in the absence of detergents and exogenous ligands to ensure advanced performance in ligand-binding assays and coregulator displacement studies.
50 mM Tris-HCl pH 8.0, 150 mM NaCl,
0.5 mM EDTA, 3 mM Dithiothreitol,
7 mM reduced glutathione, 20% glycerol.
Ligand binding and coactivator
peptide recruitment assays.
Handling upon arrival
Immediately store at -80°C.
Avoid freeze-thaw cycles.
While working, please keep
sample on ice.
Fig. 1. SDS-PAGE analysis of purified RAR-related
orphan receptor alpha LBD.
Proteins were separated in 4-20% SDS-PAGE and stained
with Coomassie blue.
2. Low range molecular weight marker (Bio-Rad).