AB Vector - Protein folding diseases

  Fig. 1. Expression of Tau isoforms.
Spodoptera frigiperda Sf9 cells were infected with negative control, a recombinant baculovirus which does not express foreign proteins or polyhedrin (lane 1), or with recombinant baculoviruses expressing different Tau isoforms, i.e. 352 a.a. isohorm (lane 2), 383 a.a. isoform (lane 3), 381 a.a. isoform (lane 4), 452 a.a. isoform K267-E mutant (lane 5), canonical 452 a.a. isoform (lane 6), 452 a.a. isoform T39-R mutant (lane 7). Cells were harvested at 60 h post infection, lysed in 50 mM Tris-HCl pH 8.0, 150 mM NaCl, 5 mM EDTA, 0.5% NP-40, and insoluble fraction was removed by centrifugation at 30.000g for 15 min. Soluble proteins were separated in 4-12% NuPAGEŽ Bis-Tris gel with MES buffer (Invitrogen) and stained with Coomassie blue.