AB Vector - PPARα-LBD
 
    PPARα-LBD Cat. #N1C1
  Purified GST-tagged human Peroxisome Proliferative Activated Receptor alpha LBD
 
  GST-tagged Peroxisome Proliferative Activated Receptor alpha ligand-binding domain (PPARα-LBD) consists of a 218 a.a. GST tag (Schistosoma japonicum Glutathione S-Transferase, Acc# 1M99_A), a 14 a.a. flexible linker and a 292 a.a. PPARα-LBD, Acc# NP_001001928.1, amino acids 177-468 (details).

Human Peroxisome Proliferative Activated Receptor alpha ligand-binding domain (PPARα-LBD) was produced in Sf9 insect cells infected with a recombinant baculovirus which encodes GST-tagged human Peroxisome Proliferative Activated Receptor alpha LBD.

GST tagged PPARα-LBD was purified using affinity chromatography to near homogeneity (Fig. 1). PPARα-LBD was produced in the absence of detergents and exogenous ligands to ensure advanced performance in ligand binding and coactivator peptide recruitment assays.

  Storage buffer
20 mM Na-P pH 8.0, 40 mM KCl,
20 mM Na2MoO4, 7 mM reduced
glutathione, 15% glycerol.

Purity >90%

Applications
Ligand binding and coactivator
peptide recruitment assays.

Handling upon arrival
Immediately store at -80C.
Avoid freeze-thaw cycles.
While working, please keep
sample on ice.






  Fig. 1. SDS-PAGE analysis of purified human Peroxisome
  Proliferative Activated Receptor alpha LBD.
  Proteins were separated in 4-20% SDS-PAGE and stained with
  Coomassie blue. Identity of PPARα-LBD was confirmed
  using mass-spectrorometry. Open the link above for
  amino acid sequence coverage.
          1.        Peroxisome Proliferative Activated Receptor alpha LBD.
          2.        Low range molecular weight marker (Bio-Rad).