AB Vector - Multi-protein expression vector

  Multi-protein expression vector
 
  A need to express several proteins in the same cells exists if the proteins have to interact to form a functional multi-protein complex or are part of an enzymatic cascade.
     pAcAB3 plasmid transfer vector can provide a high level of expression for up to 3 target proteins using the same recombinant baculovirus. Three genes encoding proteins of interest are sequentially inserted into unique sites of the vector to be expressed under the control of strong, very late baculovirus promoters. This takes more time than simultaneous construction of 3 single recombinant plasmid constructs. However, it pays off as performance of the triple recombinant baculovirus far exceeds performance of 3 single recombinant baculoviruses co-infecting host cells. Click on TECHNOLOGY for details.
     Insertion of 3 genes into the pAcAB3 vector requires only basic E.coli cloning skills. The next step, co-transfection of the resulting triple recombinant plasmid construct with linearized baculovirus DNA is also routine and the same as with other plasmid transfer vectors.

pAcAB3 plasmid transfer vector can be combined with any of the listed below linearized baculovirus DNA vectors, i.e. ProEasy™, ProGreen™, ProFold™-ER1, ProFold™-C2, ProFold™-PDI, and ProFold™-0. Click on TECHNOLOGY for more information on baculovirus expression system. Go to BACULOVIRUS TUTORIAL for simple on-line instructions on baculovirus transfection, propagation of recombinant baculoviruses and recombinant baculovirus protein expression studies.
 
  Relevant products
 
Plasmid transfer vectors                             Click on the product name for technical information!
Cat # Product Specification Unit size Price*
B2 pAcAB3 High level expression of up to 3 target proteins using a single recombinant
    baculovirus
50 μl, 0.5 μg/μl $395.00
 
Linearized baculovirus vector DNA
CsCl purified and digested with Bsu 36.I                  Click on the product name for technical information!
Cat # Product Specification Unit size Price*
A1 ProGreen™ High level of target protein expression
GFP marker to monitor virus propagation
25 μl, 2.5 μg
5 transfections
$295.00
A3 ProFold™-C2 Overexpressed Hsp40 and Hsc70 chaperones facilitate target protein folding in the      cytoplasm
GFP marker to monitor virus propagation
Slightly reduced level of protein expression
25 μl, 2.5 μg
5 transfections
$695.00
A4 ProFold™-ER1 Overexpressed calreticulin facilitates glycoprotein folding in the ER
Overexpressed PDI facilitates folding of cysteine-rich proteins
GFP marker to monitor virus propagation
High level of target protein expression
25 μl, 2.5 μg
5 transfections
$695.00
A7 ProFold™-PDI Overexpressed PDI facilitates folding of cysteine-rich proteins
High level of target protein expression from the same genome as PDI
25 μl, 2.5 μg
5 transfections
$465.00
A9 ProFold™-0 The best value baculovirus vector
High level of target protein expression
25 μl, 2.5 μg
5 transfections
$195.00
A10S ProEasy™ The easiest generation of recombinant baculoviruses
Ideal for HTP applications
High level of target protein expression
25 μl, 2.5 μg
5 transfections
$599.00
 
Controls                                                                    Click on the product name for technical information!
Cat # Product Specification Unit size Price*
C13 NC (negative control) Recombinant baculovirus stock Extracts of cells infected with NC is an ideal negative control as there is no      polyhedrin or target protein expression
NC extracts are run side by side in SDS-PAGE with extracts of cells infected with      recombinant baculoviruses
Recombinant protein is detected as a prominent band which is absent in NC      extracts
1 ml, 108 p.f.u./ml $195.00
C14 GC (green control) Recombinant baculovirus stock Positive control baculovirus in experiments where GFP fluorescence is used to      monitor virus propagation, optimize cultivation conditions, or titrate of virus stocks 1 ml, 108 p.f.u./ml $195.00
C15 GCP (green control plasmid) Plasmid transfer vector DNA Positive control for transfection with linearized GFP- baculovirus DNA. Green      fluorescence of cells is indicative of successful transfection.
50 μl, 0.2 μg/μl $145.00
 
  *Go to PRODUCTS to order online. Alternatively, email an order as pdf attachment to info@abvector.com, or send it by Fax to 1-760-454-2465 or call 1-866-683-2867 to order by phone.
 
  If you have not worked with recombinant baculoviruses, you can get a quick update at TECHNOLOGY. Go to BACULOVIRUS TUTORIAL for simple on-line instructions on baculovirus transfection, propagation of recombinant baculoviruses and recombinant baculovirus protein expression studies.
 
  Click on PRODUCTS to view the entire list of recombinant baculovirus-related products.


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