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Alternative names: Colony stimulating factor 2 (CSF-2), Macrophage-Granulocyte Inducer-1GM (MGI-1GM), pluripoietin-alpha, Sargramostim, Molgramostin.
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Catalog No: #GFA2, #GFA10, #GFA100, #GFA1m
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Background: GM-CSF is a growth factor that stimulates generation of granulocytes, macrophages, and dendritic cells from hematopoietic progenitor cells (more).
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Source: Proprietary GM-CSF superproducing E.coli strain (more).
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Formulation: Lyophilized purified fraction (>97% pure by SDS-PAGE) without additives.
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Sterility: Sterile-filtered through 0.22 micron filter prior to lyophilization.
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Reconstitution: Restore in water to a concentration of 0.1-1.0 mg/ml. This solution can be stored at 2-8oC for 1 week or aliquoted and kept frozen at -20oC for future use. Further dilutions should be made into a medium containing serum or into PBS supplemented with 0.1% BSA, which can be stored at -20oC. Avoid repeated freezing and thawing.
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Description: Recombinant human GM-CSF is a globular protein consisting of 128 amino acids containing two intramolecular disulfide bonds.
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Amino Acid Sequence: MAPARSPSPS TQPWEHVNAI QEARRLLNLS RDTAAEMNET VEVISEMFDL QEPTCLQTRL ELYKQGLRGS LTKLKGPLTM MASHYKQHCP PTPETSCATQ IITFESFKEN LKDFLLVIPF DCWEPVQE
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Molecular weight: 14 kDa.
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Biological activity: The ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is < 0.1 ng/ml.
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Endotoxin level: < 0.01 ng/μg (0.1 IEU/μg) of GM-CSF.
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