AB Vector - His tag vectors

  His tag vectors
 
  The polyhistidine tag is one of the most widely used tags. It can be provided on the N- or C-terminus of target proteins and can be used for protein purification by immobilized metal affinity chromatography and for immunodetection. Target proteins can be provided with a cleavable N-terminal 6xHis tag using our pAB-6xHis™ vector, or with a C-terminal FLAG-8xHis tag using pVL-FH vector.

The pAB-6xHis-MBP™ vector provides a cleavable N-terminal 6xHis-MBP tag. MBP (maltose binding protein) moiety is well documented to increase yield and solubility of target fusion proteins. Furthermore, it ensures exposure of the 6xHis tag on the surface of the fusion protein, which facilitates affinity purification under native conditions.

Another vector, pAB-bee™-8xHis is ideal for the expression of polyhistidine-tagged proteins which are translocated into the endoplasmic reticulum (ER), such as secreted and transmembrane proteins. Using this vector, the target protein can be provided with a strong signal sequence to ensure its efficient translocation into the ER, followed by a signal peptidase cleavage site and an N-terminal 8xHis tag. Optionally the 8xHis tag can be also provided on the C-terminus of a target protein. A similar pAB-bee vector encodes a strong signal sequence and an 8xHis tag which is provided on the C- terminus of the translocated proteins, but it does not encode for the N-terminal tag. A derivative of this vector pAB-bee™-FH encodes strong signal sequence and C-terminal dual FLAG-8xHis tag.

The plasmid transfer vectors described above can be combined with any of the listed below linearized baculovirus DNA vectors, i.e. ProEasy™, ProGreen™, ProFold™-ER1, ProFold™-C2, ProFold™-PDI, and ProFold™-0. Click on TECHNOLOGY for more information on baculovirus expression system. Go to BACULOVIRUS TUTORIAL for simple on-line instructions on baculovirus transfection, propagation of recombinant baculoviruses and recombinant baculovirus protein expression studies.
 
  Relevant products
 
Plasmid transfer vectors*                             Click on the product name for technical information!
Cat # Product Specification Unit size Price**
B3 pAB-bee Provides target proteins with powerful honeybee melittin signal sequence to      facilitate translocation into the ER
Recommended for high level expression of secreted and transmembrane proteins
50 μl, 0.5 μg/μl $495.00
B3H pAB-bee™-8xHis Provides target proteins with powerful honeybee melittin signal sequence to      facilitate translocation into the ER
Recommended for high level expression of secreted and transmembrane proteins
N- or C-terminal 8xHis tag for affinity purification
50 μl, 0.5 μg/μl $495.00
B3FH pAB-bee™-FH Provides target proteins with powerful honeybee melittin signal sequence to      facilitate translocation into the ER
Recommended for high level expression of secreted and transmembrane proteins
C-terminal FLAG-8xHis tag for affinity purification and immonodetection
50 μl, 0.5 μg/μl $495.00
B4 pAB-6xHis™ High level expression of proteins with cleavable N-terminal 6xHis tag 50 μl, 0.5 μg/μl $295.00
B7 pAB-6xHis-MBP™ High level expression of proteins with cleavable N-terminal 6xHis MBP tag
High level expression of proteins with C-terminal MBP tag
MBP tag improves protein solubility and yield
50 μl, 0.5 μg/μl $495.00
B9 pVL-FH High level expression of proteins with C-terminal FLAG-8xHis tag 50 μl, 0.5 μg/μl $295.00
 
Linearized baculovirus vector DNA
CsCl purified and digested with Bsu 36.I                  Click on the product name for technical information!
Cat # Product Specification Unit size Price**
A1 ProGreen™ High level of target protein expression
GFP marker to monitor virus propagation
25 μl, 2.5 μg
5 transfections
$295.00
A3 ProFold™-C2 Overexpressed Hsp40 and Hsc70 chaperones facilitate target protein folding in the      cytoplasm
GFP marker to monitor virus propagation
Slightly reduced level of protein expression
25 μl, 2.5 μg
5 transfections
$695.00
A4 ProFold™-ER1 Overexpressed calreticulin facilitates glycoprotein folding in the ER
Overexpressed PDI facilitates folding of cysteine-rich proteins
GFP marker to monitor virus propagation
High level of target protein expression
25 μl, 2.5 μg
5 transfections
$695.00
A7 ProFold™-PDI Overexpressed PDI facilitates folding of cysteine-rich proteins
High level of target protein expression from the same genome as PDI
25 μl, 2.5 μg
5 transfections
$465.00
A9 ProFold™-0 The best value baculovirus vector
High level of target protein expression
25 μl, 2.5 μg
5 transfections
$195.00
A10S ProEasy™ The easiest generation of recombinant baculoviruses
Ideal for HTP applications
High level of target protein expression
25 μl, 2.5 μg
5 transfections
$599.00
 
Controls                                                                    Click on the product name for technical information!
Cat # Product Specification Unit size Price**
C13 NC (negative control) Recombinant baculovirus stock Extracts of cells infected with NC is an ideal negative control as there is no      polyhedrin or target protein expression
NC extracts are run side by side in SDS-PAGE with extracts of cells infected with      recombinant baculoviruses
Recombinant protein is detected as a prominent band which is absent in NC      extracts
1 ml, 108 p.f.u./ml $195.00
C14 GC (green control) Recombinant baculovirus stock Positive control baculovirus in experiments where GFP fluorescence is used to      monitor virus propagation, optimize cultivation conditions, or titrate of virus stocks 1 ml, 108 p.f.u./ml $195.00
C15 GCP (green control plasmid) Plasmid transfer vector DNA Positive control for transfection with linearized GFP- baculovirus DNA. Green      fluorescence of cells is indicative of successful transfection.
50 μl, 0.2 μg/μl $145.00
 
  *The above plasmid transfer vectors are compatible with linearized baculovirus DNA from several other suppliers, e.g. BacMagic™, BacMagic™-2, BacMagic™-3, BacVector™-1000, BacVector™-2000, BacVector™-3000 (Novagen); BaculoGold™, BaculoGold™ bright (BD Pharmingen); flashBACGOLD™, flashBACULTRA™, flashBAC™ (OET); BacPAK6™ (Clontech); Sapphire™ (Orbigen). They are not compatible with Bac-to-Bac® and BaculoDirect™ DNA from Invitrogen. BacMagic™, BacMagic™-2, BacMagic™-3, BacVector™-1000, BacVector™-2000, BacVector™-3000 are trademarks of Novagen; BaculoGold™, BaculoGold™ bright are trademarks of BD Pharmingen; flashBACGOLD™, flashBACULTRA™, flashBAC™ are trademarks of OET; BacPAK6™ is a trademark of Clontech; Sapphire™ is a trademark of Orbigen, Bac-to-Bac® and BaculoDirect™ are trademarks of Invitrogen.

  **Go to PRODUCTS to order online. Alternatively, email an order as pdf attachment to info@abvector.com, or send it by Fax to 1-760-454-2465 or call 1-866-683-2867 to order by phone.
 
  If you have not worked with recombinant baculoviruses, you can get a quick update at TECHNOLOGY. Go to BACULOVIRUS TUTORIAL for simple on-line instructions on baculovirus transfection, propagation of recombinant baculoviruses and recombinant baculovirus protein expression studies.
 
  Click on PRODUCTS to view the entire list of recombinant baculovirus-related products.


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