AB Vector - FoldHelper™-37


 

FoldHelper™-37 Cat. #H18

 

 

FoldHelper™-37 is a recombinant baculovirus, which provides for the expression of human Cdc37 (Acc# Q16543) under control of a strong polyhedrin promoter. Cdc37 is found in soluble protein fraction of lysates of Sf9 cells infected with FoldHelper™-37 and is expressed to a high level (Fig. 1 ). Cdc37 expressed in baculovirus-insect cell system is phosphorylated and migrates in SDS-PAGE as a broad band that becomes prominent at over 60 h post-infection. Similarly to Cdc37 expressed in mammalian cells, human Cdc37 expressed in insect cells using recombinant baculoviruses is phosphorylated on Ser13 that is required for activation of protein kinase clients by Hsp90-Cdc37 (Vaughan C. K. et al., Molecular Cell, 23: 697-707, 2006).

 

 

Fig. 1. Expression of Cdc37.Spodoptera frigiperda Sf9 cells were infected
with FoldHelper™-37 (lane 1) or negative control,
a recombinant baculovirus which does not express
foreign proteins or polyhedrin (lane 2).
Cells were harvested at 60 h post infection,
lysed in 50 mM Tris-HCl pH 8.0, 150 mM NaCl, 5 mM EDTA,
0.5% NP-40, and insoluble fraction was removed
by centrifugation at 30,000 g for 15 min. Soluble
proteins were separated in 10% SDS-PAGE and
stained with Coomassie blue.

 

 

Cdc37 is a co-chaperone that binds to numerous kinases and promotes their interaction with the Hsp90 complex, resulting in stabilization and promotion of their activity. Cdc37 also displays a variety of Hsp90-independent activities and can function as a molecular chaperone by itself (McLean M. and Picard D., Cell Stress & Chaperones, 8:114-119, 2003). Cdc37 interacts with Androgen receptor CDK4, CDK6, EIF2AK1 and RB1. A comprehensive list of Cdc37 interacting proteins can be found on Dr. Picard's web site (http://www.picard.ch/downloads/Cdc37interactors.pdf ).

 

 

Recommended use: co-infection with recombinant baculoviruses expressing Cdc37-interacting proteins.

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