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Overexpressed cysteine-rich proteins often aggregate due to the incorrect formation of disulfide bonds. In unmodified cells these proteins do not aggregate since they are expressed at low level (typically below 0.1% of the total cell protein). At such low concentrations molecular chaperones are in excess to the cysteine-rich proteins which ensures correct formation of disulfide bonds. However, if a cysteine-rich protein is overexpressed, its concentration by far exceeds the concentration of molecular chaperones, resulting in its misfolding and aggregation.
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AB Vector's insect cell-baculovirus expression systems allow overexpression of both a cysteine-rich protein and the molecular chaperone protein disulfide isomerase (PDI), which is responsible for the correct formation of disulfide bonds. |
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1.For best results co-express cysteine-rich target protein and PDI using the same recombinant baculovirus. To this end, clone a DNA fragment encoding the target protein into a plasmid transfer vector, such as pVL1393 and co-transfect the resulting construct with ProFold™-PDI linearized baculovirus DNA.
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2.You have already constructed a recombinant baculovirus using conventional vectors, but you would like to supplement expression of target with expression of PDI. To this end, co-infect insect cells with your recombinant baculovirus and FoldHelper™-57P, which expresses PDI to a high level.
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3.Target cysteine-rich protein is a glycoprotein. In this case follow recommendations for Glycoproteins.
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4.Target cysteine-rich protein is a secreted protein. In this case follow recommendations for Secreted proteins.
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5.Target cysteine-rich protein is a transmembrane protein. In this case follow recommendations for Transmembrane proteins.
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Plasmid transfer vectors
Click on the product name for technical information!
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B1 |
pVL1393 |
High level of target protein expression |
50 μl, 0.5 μg/μl |
$340.00
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Linearized baculovirus vector DNA
CsCl purified and digested with Bsu 36.I
Click on the product name for technical information!
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A7 |
ProFold™-PDI |
Overexpressed PDI facilitates folding of cysteine-rich proteins
High level of target protein expression from the same genome as PDI |
25 μl, 2.5 μg 5 transfections |
$830.00
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A10S |
ProEasy™ |
The easiest generation of recombinant baculoviruses
Ideal for HTP applications
High level of target protein expression |
25 μl, 2.5 μg 5 transfections |
$695.00
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FoldHelper™ recombinant baculovirus
Click on the product name for technical information!
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H17 |
FoldHelper™-57PRecombinant baculovirus stock |
Overexpressed PDI and ERp57 to facilitate folding of cysteine-rich proteins |
1 ml, 108 p.f.u./ml |
$295.00
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*Go to PRODUCTS to order online. Alternatively, email an order as pdf attachment to info@abvector.com, or send it by Fax to 1-760-454-2465 or call 1-866-683-2867 to order by phone.
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If you have not worked with baculovirus expression system, also referred to as baculovirus expression vector system or BEVS, you can get a quick update at TECHNOLOGY. Go to BACULOVIRUS TUTORIAL for simple on-line instructions on baculovirus transfection, propagation of recombinant baculoviruses and recombinant baculovirus protein expression studies |
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Click on PRODUCTS to view the entire list of recombinant baculovirus-related products. |